اثر هشت هفته تمرین ورزشی شدت متوسط بر بیان ژنی آنزیم مبدل آنژیوتانسین و فعالیت آنژیوتانسین-2 در مردان میانسال

  Background : The aim of this research is investigation of angiotensin converting enzyme gene expression and angiotensin II activity in middle-aged men , following to eight weeks moderate exercise.   Methods: This study was a semi-experimental research with a repeated measures design. From 96 volunteer middle-aged men, 20 subject (age range of 45-55 years) participated in this study after signing an informed consent form. Next, subject divided in two groups of training (10 person) and control (10) groups and performed the eight weeks moderate exercise training (time: 45 minutes, speed: 50-65 maximal heart rate, slope: 0%). Blood samples were collected in three times: baseline, after 4 week and after 8 week, and Real time- PCR ) Polymerase Chain Reaction ( was used for evaluation of Angiotensin Converting Enzyme (ACE) mRNA and Elisa methods for angiotensin II (Ang II).   Results: AngII activity in training group were not significantly changed after 4 weeks exercise training (p=0.255) but significantly increased after 8 weeks (p= 0.004). In control groups AngII increased after 4 and 8 weeks exercise training (respectively p=0.952 and p=0.876). ACE gene expression was significantly reduced in training group after 4 and 8 weeks (p=0.001), but in control group, ACE gene expression was not increased after 4 weeks of no regular exercises (p=0.35), but after 8 weeks, ACE mRNA significantly increased (p=0.001).   Conclusion: Moderate exercise training has reduced the genetic and blood markers of blood pressure in middle-aged men, but not doing regular exercises increased this factor.